Monday, May 20, 2013

Food laboratory accuracy remains a concern

Food laboratory accuracy remains a concern [ Back to EurekAlert! ] Public release date: 20-May-2013
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Contact: Jim Sliwa
jsliwa@asmusa.org
202-942-9297
American Society for Microbiology

Food microbiology laboratories continue to submit false negative results and false positive results on a routine basis. A retrospective study of nearly 40,000 proficiency test results over the past 14 years, presented today at the 113th General Meeting of the American Society for Microbiology, examined the ability of food laboratories to detect or rule out the presence of Escherichia coli O157:H7, Salmonella, Listeria monocytogenes, and Campylobacter.

"There is concern when laboratories report that pathogens are not found in a food sample, when in fact they are there," explained Christopher Snabes, lead author on the study. "This is known as a 'false negative'. Similar concerns arise when a laboratory reports a 'false positive' suggesting that pathogens are in the food sample, when indeed they are not."

The study found that, on average, food laboratories report false negatives of 9.1% for Campylobacter, a bacterial foodborne illness that may cause bloody diarrhea, cramping and fever, and 4.9% for Salmonella, a bacteria that may cause diarrhea, fever and abdominal cramps sometimes leading to hospitalization or death. The false positive rate, on average, is 3.9% for Salmonella, and 2.5% for both E. coli and L. monocytogenes.

This study was conducted by the American Proficiency Institute (API) located in Traverse City, Michigan. API is a private institute that supplies proficiency testing programs for food laboratories and clinical laboratories.

API offers proficiency testing (PT) as an objective method for measuring the accuracy of a laboratory. Participants use API PT up to three times a year to examine the accuracy of their laboratory personnel and their testing methods. The purpose of PT is to determine if the laboratory professional can properly respond to API with correct answers as to what API places in a food sample. PT may test for presence or absence of a substance in a qualitative test, and sometimes PT may require an enumeration response, or quantitative test.

Currently, food laboratories are not required to assess the accuracy or quality of their tests. Laboratories that utilize API PT are doing so voluntarily. Some laboratories use API services to obtain and maintain accreditation. API food microbiology PT programs are used by over 700 food laboratories in 43 countries.

Proficiency testing is an objective means for measuring laboratory accuracy. "Improved accuracy in our nation's food laboratories will lead to a safer food supply," noted Snabes.

The Food Safety Modernization Act, passed in 2011, included sweeping changes to the country's food safety requirements. Model laboratory standards and laboratory accreditation are addressed as important components of the law. Once rules are promulgated, it is anticipated that all food laboratories will need to ensure that their personnel, and the test methods they use, are in compliance with the law. Yet, food laboratories may start using proficiency testing now to help ensure a safer food product.

###

This research was presented as part of the 2013 General Meeting of the American Society for Microbiology held May 18-21, 2013 in Denver, Colorado. A full press kit for the meeting, including tipsheets and additional press releases, can be found online at http://bit.ly/asm2013pk.

The American Society for Microbiology is the largest single life science society, composed of over 39,000 scientists and health professionals. ASM's mission is to advance the microbiological sciences as a vehicle for understanding life processes and to apply and communicate this knowledge for the improvement of health and environmental and economic well-being worldwide.


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Food laboratory accuracy remains a concern [ Back to EurekAlert! ] Public release date: 20-May-2013
[ | E-mail | Share Share ]

Contact: Jim Sliwa
jsliwa@asmusa.org
202-942-9297
American Society for Microbiology

Food microbiology laboratories continue to submit false negative results and false positive results on a routine basis. A retrospective study of nearly 40,000 proficiency test results over the past 14 years, presented today at the 113th General Meeting of the American Society for Microbiology, examined the ability of food laboratories to detect or rule out the presence of Escherichia coli O157:H7, Salmonella, Listeria monocytogenes, and Campylobacter.

"There is concern when laboratories report that pathogens are not found in a food sample, when in fact they are there," explained Christopher Snabes, lead author on the study. "This is known as a 'false negative'. Similar concerns arise when a laboratory reports a 'false positive' suggesting that pathogens are in the food sample, when indeed they are not."

The study found that, on average, food laboratories report false negatives of 9.1% for Campylobacter, a bacterial foodborne illness that may cause bloody diarrhea, cramping and fever, and 4.9% for Salmonella, a bacteria that may cause diarrhea, fever and abdominal cramps sometimes leading to hospitalization or death. The false positive rate, on average, is 3.9% for Salmonella, and 2.5% for both E. coli and L. monocytogenes.

This study was conducted by the American Proficiency Institute (API) located in Traverse City, Michigan. API is a private institute that supplies proficiency testing programs for food laboratories and clinical laboratories.

API offers proficiency testing (PT) as an objective method for measuring the accuracy of a laboratory. Participants use API PT up to three times a year to examine the accuracy of their laboratory personnel and their testing methods. The purpose of PT is to determine if the laboratory professional can properly respond to API with correct answers as to what API places in a food sample. PT may test for presence or absence of a substance in a qualitative test, and sometimes PT may require an enumeration response, or quantitative test.

Currently, food laboratories are not required to assess the accuracy or quality of their tests. Laboratories that utilize API PT are doing so voluntarily. Some laboratories use API services to obtain and maintain accreditation. API food microbiology PT programs are used by over 700 food laboratories in 43 countries.

Proficiency testing is an objective means for measuring laboratory accuracy. "Improved accuracy in our nation's food laboratories will lead to a safer food supply," noted Snabes.

The Food Safety Modernization Act, passed in 2011, included sweeping changes to the country's food safety requirements. Model laboratory standards and laboratory accreditation are addressed as important components of the law. Once rules are promulgated, it is anticipated that all food laboratories will need to ensure that their personnel, and the test methods they use, are in compliance with the law. Yet, food laboratories may start using proficiency testing now to help ensure a safer food product.

###

This research was presented as part of the 2013 General Meeting of the American Society for Microbiology held May 18-21, 2013 in Denver, Colorado. A full press kit for the meeting, including tipsheets and additional press releases, can be found online at http://bit.ly/asm2013pk.

The American Society for Microbiology is the largest single life science society, composed of over 39,000 scientists and health professionals. ASM's mission is to advance the microbiological sciences as a vehicle for understanding life processes and to apply and communicate this knowledge for the improvement of health and environmental and economic well-being worldwide.


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AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.


Source: http://www.eurekalert.org/pub_releases/2013-05/asfm-fla051613.php

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Victims: Marines failed to safeguard water supply

CAMP LEJEUNE, N.C. (AP) ? A simple test could have alerted officials that the drinking water at Camp Lejeune was contaminated, long before authorities determined that as many as a million Marines and their families were exposed to a witch's brew of cancer-causing chemicals.

But no one responsible for the lab at the base can recall that the procedure ? mandated by the Navy ? was ever conducted.

The U.S. Marine Corps maintains that the carbon chloroform extract (CCE) test would not have uncovered the carcinogens that fouled the southeastern North Carolina base's water system from at least the mid-1950s until wells were capped in the mid-1980s. But experts say even this "relatively primitive" test ? required by Navy health directives as early as 1963 ? would have told officials that something was terribly wrong beneath Lejeune's sandy soil.

A just-released study from the federal Agency for Toxic Substances and Disease Registry cited a February 1985 level for trichloroethylene of 18,900 parts per billion in one Lejeune drinking water well ? nearly 4,000 times today's maximum allowed limit of 5 ppb. Given those kinds of numbers, environmental engineer Marco Kaltofen said even a testing method as inadequate as CCE should have raised some red flags with a "careful analyst."

"That's knock-your-socks-off level ? even back then," said Kaltofen, who worked on the infamous Love Canal case in upstate New York, where drums of buried chemical waste leaked toxins into a local water system. "You could have smelled it."

Biochemist Michael Hargett agrees that CCE, while imperfect, would have been enough to prompt more specific testing in what is now recognized as the worst documented case of drinking-water contamination in the nation's history.

"I consider it disingenuous of the Corps to say, 'Well, it wouldn't have meant anything,'" said Hargett, co-owner of the private lab that tried to sound the alarm about the contamination in 1982. "The levels of chlorinated solvent that we discovered ... they would have gotten something that said, 'Whoops. I've got a problem.' They didn't do that."

Trichloroethylene (TCE), tetrachloroethylene (PCE), benzene and other toxic chemicals leeched into ground water from a poorly maintained fuel depot and indiscriminate dumping on the base, as well as from an off-base dry cleaner.

Nearly three decades after the first drinking-water wells were closed, victims are still awaiting a final federal health assessment ? the original 1997 report having been withdrawn because faulty or incomplete data. Results of a long-delayed study on birth defects and childhood cancers were only submitted for publication in late April.

Many former Lejeune Marines and family members who lived there believe the Corps still has not come clean about the situation, and the question of whether these tests were conducted is emblematic of the depth of that mistrust.

Marine Corps officials have repeatedly said that federal environmental regulations for these cancer-causing chemicals were not finalized under the Safe Drinking Water Act until 1989 ? about four years after the contaminated wells had been identified and taken out of service. But victims who have scoured decades-old documents say the military's own health standards should have raised red flags long before.

In 1963, the Navy's Bureau of Medicine and Surgery issued "The Manual of Naval Preventive Medicine." Chapter 5 is titled "Water Supply Ashore."

"The water supply should be obtained from the most desirable sources which is feasible, and effort should be made to prevent or control pollution of the source," it reads.

At the time, the Defense Department adopted water quality standards set by the U.S. Public Health Service. To measure that quality, the Navy manual identified CCE "as a technically practical procedure which will afford a large measure of protection against the presence of undetected toxic materials in finished drinking water."

Also referred to as the "oil and grease test," CCE was intended to protect against an "unwarranted dosage of the water consumer with ill-defined chemicals," according to the Navy manual. The CCE standard set in 1963 was 200 ppb. In 1972, the Navy further tightened it to no more than 150 ppb.

In response to a request from The Associated Press, Capt. Kendra Motz said the Marines could produce no copies of CCE test results for Lejeune, despite searching for "many hours."

"Some documents that might be relevant to your question may no longer be maintained by the Marine Corps or the Department of the Navy in accordance with records management policies," she wrote in an email. "The absence of records 50 years later does not necessarily mean action was not taken."

But the two men who oversaw the base lab told the AP they were not even familiar with the procedure.

"A what?" asked Julian Wooten, who was head of the Lejeune environmental section during the 1970s, when asked if his staff had ever performed the CCE test. "I never saw anything, unless the (Navy's) preventive medicine people were doing some. I don't have any knowledge of that kind of operation or that kind of testing being done. Not back then."

"I have no knowledge of it," said Danny Sharpe, who succeeded Wooten as section chief and was in charge when the first drinking water wells were shut down in the mid-1980s. "I don't remember that at all."

Wooten was an ecologist, and Sharpe's background is in forestry and soil conservation. But Elizabeth Betz, the supervisory chemist at Lejeune from 1979 to 1995, was also at a loss when asked about the CCE testing.

"I do not remember any such test being requested nor do I remember seeing any such test results," Betz, who later worked for the U.S. Environmental Protection Agency's national exposure branch at Research Triangle Park outside Raleigh, wrote in a recent email.

Hargett, the former co-owner of Grainger Laboratories in Raleigh, said he never saw any evidence that the base was testing and treating for anything beyond e coli and other bacteria.

"That was a state regulation ... that they had to maintain a sanitary water supply," he said. "And they did a good job at that."

Motz, the Marine spokeswoman, told the AP that the method called for in the manual would not have detected the toxins at issue in the Camp Lejeune case.

"The CCE method includes a drying step and a distillation (evaporation) step where chloroform is completely evaporated," she wrote in an email. These volatile organic compounds, "by their chemical nature, would evaporate readily as well," she wrote.

ATSDR contacted the EPA about the "utility" of such testing and concluded it would be of no value in detecting TCE, PCE, or benzene, Deputy Director Tom Sinks wrote in an email to members of a community assistance panel on Lejeune.

"It is doubtful that the weight of their residue would be detectable when subjected to this method," Sinks wrote.

Kaltofen, a doctoral candidate at Worcester Polytechnic Institute in Massachusetts, acknowledged that CCE is "a relatively primitive test." But in addition to the water's odor, Kaltofen said, "there are some things that a careful analyst would easily have noticed."

Hargett agreed.

"It would have prompted you to simply say, 'Wow. There is something here. Let's do some additional work,'" he told the AP. Any "reputable chemist ... would have raised their hands to the person responsible and said, 'Guys. You ought to look at this. There's more here.'"

The Marines have said such high readings were merely spikes. But Kaltofen countered that, "You can't get that level even once without having a very serious problem ... It's the worst case."

In a recent interview, Wooten told the AP that he knew something was wrong with the water as early as the 1960s, when he worked in the maintenance department.

"I was usually the first person in in the big building that we worked in," he said. "And I'd cut the water on and let it run, just go and flush the commodes and cut the water on and let it run for several minutes before I'd attempt to make coffee."

Wooten said he made repeated budget requests for additional equipment and lab workers. But as Betz told a federal fact-finding group, "the lab was very low on the priority list at the base."

She said her group ? the Natural Resources and Environmental Affairs Department ? was "like the 'red headed stepchild.'"

Even a series of increasingly urgent reports from an Army lab at Fort McPherson, Ga., beginning in late 1980, failed to prompt any real action.

"WATER HIGHLY CONTAMINATED WITH OTHER CHLORINATED HYDROCARBONS (SOLVENTS!)" cautioned one memo from the Army lab in early 1981.

Because the base water system drew on a rotating basis from a number of different wells, subsequent tests showed no problems, and officials chalked these "interferences" up to flukes. One base employee told the fact-finding group that in 1980, "they simply did not have the money nor capacity" to test every drinking-water well on the base.

"This type of money would have cost well over $100,000, and their entire operating budget was $100,000," the employee said, according to a heavily redacted summary obtained by the AP from the Department of Justice through the Freedom of Information Act. "However, they did not do the well testing because they did not think they needed to."

So, from late 1980 through the summer of 1982, the former employee told investigators, "this issue simply laid there. No attempts were made to identify ground contamination" at Hadnot Point or Tarawa Terrace, where most of the enlisted men and their families lived.

It wasn't until a letter from Grainger in August 1982 reported TCE levels of 1,400 ppb that any kind of widespread testing began. Though the EPA did not yet enforce a limit for TCE at the time, the chemical had long been known to cause serious health problems.

"That is when the light bulb went off," Sharpe told federal investigators in a 2004 interview, obtained by the AP. "That is when we connected the tests of the 1980, 1981, and 1982 time period where traces of solvents were detected to this finding."

Still, it was not until the final weeks of 1984 that the first wells were closed down. Between the receipt of that 1982 letter and the well closures, the employee told the fact-finding group, "they simply dropped the ball."

Each year of delay meant an additional 10,000 people may have been exposed, according to Marine estimates.

Municipal utilities around the country were using far more sophisticated tests to detect much lower contaminate levels, said Kaltofen, while the people at Camp Lejeune were doing "the bare minimum. And it wasn't enough."

Last year, President Obama signed the Camp Lejeune Veterans and Family Act to provide medical care and screening for Marines and their families, but not civilians, exposed between 1957 and 1987 ? although preliminary results from water modeling suggest that date be pushed back at least another four years. The law covers 15 diseases or conditions, including female infertility, miscarriage, leukemia, multiple myeloma, as well as bladder, breast, esophageal, kidney and lung cancer.

Jerry Ensminger, a former drill sergeant, blames the water for the leukemia that killed his 9-year-old daughter, Janey, in 1985. He and Michael Partain ? a Marine's son who is one of at least seven dozen men with Lejeune ties diagnosed with a rare form of breast cancer ? have scoured the records, and he thinks the Corps has yet to accept responsibility for its role in this tragedy.

"If I hadn't dug in my heels," Ensminger said, "this damned issue would have been dead and buried along with my child and everybody else's."

___

Online:

ATSDR's Camp Lejeune page http://www.atsdr.cdc.gov/sites/lejeune/

___

Breed, a national writer, reported from Camp Lejeune. Biesecker and Waggoner reported from Raleigh, N.C.

Follow them on Twitter at twitter.com/AllenGBreed, twitter.com/mbieseck and twitter.com/mjwaggonernc

Source: http://news.yahoo.com/victims-marines-failed-safeguard-water-supply-135139535.html

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Sunday, May 19, 2013

The South welcomes 'crazy ants.' Hail the latest invader

'Crazy ants' from South America are hitching rides across the South, setting up massive colonies, and relieving other occupying ant armies, including fire ants, of their duties.

By Patrik Jonsson,?Staff writer / May 18, 2013

Hairy crazy ants are on the move in Florida, Texas, Mississippi, and Louisiana. The flea-sized critters are called crazy because each ant in the horde seems to scramble randomly, moving so fast that videos look as if they're on fast-forward.

Joe MacGown/Mississippi State Entomological Museum/AP

Enlarge

The South is being invaded ? again. This time it?s erratic but troublesome ?crazy ants? from South America marching ? actually, hitching rides ? across the South, setting up massive colonies, and relieving other occupying ant armies, including fire ants, of their duties.

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With billions of ants possible per acre, crazy ants, known for their random, jerky travel, eat or chase away most other insects and reptiles, and hound yard pets inside. In single numbers pretty innocuous-looking, tiny tawny crazy ants also make pests out of themselves by sometimes biting people and shorting out home electrical wiring.

The question now is if there?s room enough in the South for the newcomers, or whether the US needs to invest in research to figure out how to stop the ?tawny crazy ant,? a well as its cousins, the ?black crazy ant,? and the ?Caribbean crazy ant,? before they?re ubiquitous.

?The entire Gulf Coast is going to be inundated in a very short period of time,? entomologist Tom Rasberry, who found and identified the crazy ants in 2002, recently told a local CBS News broadcast.

Having already spread in the span of a decade from a bunch of counties surrounding Houston to as far away as Florida, crazy ant success so far is entirely due to their hitchhiking skills. A few ants clambering aboard at a truck stop in Waco, Texas, may soon find themselves starting a colony in Covington, Georgia. They thrive best in warmer, moister locales, which means the South is stuck with them.

But as with most invasive species, ?crazy ants? can have unexpected, sometimes paradoxical, impacts on their conquered ground. Fire ants, which were accidentally introduced in the 1930s and now pretty much own the South, apparently can?t stand ?crazy ants,? and retreat from the conquering horde, as do most other ant species.

And despite oftentimes legitimate cries of concern from the scientific community about the dangers of invasive plants and animals in the US, many one-time strangers-to-these-parts ? kudzu, snakeheads, boas, Yankees ? become, at least in the eyes of some Southerners, manageable pests, part of an ever-changing backdrop of wildlife at the door.

This being the South, even scientists have sought out Biblical insights into how to view the invasion, including this from Proverbs 6:6-8 cited in a Texas A&M research paper about invasive ants: ?Go to the ant, O sluggard/ Observe her ways and be wise/It has no commander/No overseer or ruler.??

Source: http://rss.csmonitor.com/~r/feeds/csm/~3/SDHmMAlOZWE/The-South-welcomes-crazy-ants.-Hail-the-latest-invader

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Suspected US drone in Yemen kills 4 militants

SANAA, Yemen (AP) ? Yemeni security officials say a suspected US drone strike killed four al-Qaida militants in the country's south.

The officials say the attack took place around dawn Saturday in an area called Deyfa in Abyan province. Officials spoke anonymously because they were not authorized to brief the media.

Yemeni forces battled al-Qaida in Abyan province last year, routing out militants from major cities that al-Qaida had overrun during the country's 2011 political turmoil. The militants fled to surrounding mountainous areas.

According to several research groups and The Associated Press's own reporting, there has been a dramatic rise in such drone strikes in Yemen since the country's new U.S.-backed president assumed power early last year.

Washington says al-Qaida in Yemen is among the group's most dangerous and active branches worldwide.

Source: http://news.yahoo.com/suspected-us-drone-yemen-kills-4-militants-193326334.html

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Big bling free to celebs in Cannes 'gifting suites'

Celebs

17 hours ago

Gather hundreds of celebrities and film executives on the French Riviera for the Cannes Film Festival and naturally, they need their glitter and glam.

One way the famous end up looking so good and so trendy is that often, they don't have to bring, or buy, their own fabulousness: They get special invitations to luxury "gifting suites" set up in hotel rooms at the film festival and can walk out with thousands of dollars of clothes and jewelry.

These rooms of swag aren't the only places where there's bling-related excitement to be found -- someone stole jewels meant for celebrities to wear on the red carpet straight from a hotel room very early on in the festival.

The Hollywood Reporter took a look inside some of these swag rooms stocked by famous brands including Chanel, Swarovski, Dior, and Jimmy Choo to find out what's up for grabs -- if you've got the right A-list name. Check out the video!

Source: http://www.today.com/entertainment/celebrities-visit-luxury-gifting-suites-cannes-walk-out-serious-swag-1C9983842

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Ellen Slams Abercrombie: Fitch, Please!

Source: http://www.thehollywoodgossip.com/2013/05/ellen-slams-abercrombie-fitch-please/

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Bears in Their Own Words: Chicago Bear Greats Talk About the ...

Bears in Their Own Words: Chicago Bear Greats Talk About the Team, the Game, the Coaches, and the Times of Their Lives book download

Bears in Their Own Words: Chicago Bear Greats Talk About the Team, the Game, the Coaches, and the Times of Their Lives Richard Whittingham

Richard Whittingham

. Get College. Football Fanatics - Sports Apparel, NFL Gear, Fan Shop, Jerseys. Topic Galleries -- OrlandoSentinel.com Topic galleries provide easy access to stories and photos about people, places, organizations, events and subjects of interest to you. Chicago Sports News: Sports News, Scores and Schedules. Heat CNN Video - Breaking News Videos from CNN.com Get unlimited access to live CNN TV and HLN TV on your computer or on the CNN Apps for iPhone® and iPad®. Bulls shoot 25.7 percent, score just 65 points to fall behind Heat 3-1 in series. 5.6.13Comets no match for South HavenBy FARAH GREENFor the TribuneThe South Haven boys track team coasted by Coloma on Tuesday, while the girls fell to the Comets in. They bring together rich. Topic galleries provide easy access to stories and photos about people, places, organizations, events and subjects of interest to you. They bring together rich. Panthers | Restructure DeAngelo Williams' deal (Wed May 15, 09:07 AM) [jump to this story] Rams | Plan to get Austin the ball early and often (Tue May 14, 09:07 PM. Chicago sports and scores from the Chicago Sun-Times Chicago sports news and scores: Bears, Blackhawks, Bulls, Cubs and White Sox NFL News, Videos, Scores, Teams, Standings, Stats - FOX Sports on MSN Live NFL scores, in-depth pro football player and team news, NFL videos, rumors, updated stats, team schedules, fantasy football games, playoff coverage, league. Video News, Highlights, and Exclusive Analysis - Yahoo! Sports You're watching the latest sports videos from Yahoo! Sports Latest News - Fantasy Football - Footballguys Fantasy Football. Shop Football Fanatics for sports apparel and fan merchandise! Our ultimate sports store features NFL Apparel, Sportswear and Gifts for every team. Topic Galleries -- chicagotribune.com - Chicago Tribune: Chicago. Sports and recreation - South Haven Tribune - HomeView the area's. Game 4 Photos: Bulls vs


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